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自然-材料学:新型干细胞培养液

摘要 : 研究人员在9月在线出版的《自然-材料学》期刊上报告,在没有添加动物和其它成分的情况下,一种取自游离细胞的人体干细胞实现了无性繁殖。这种快速、定量的方法能导致具有重要临床意义
自然-材料学:新型干细胞培养液

研究人员在9月在线出版的《自然-材料学》期刊上报告,在没有添加动物和其它成分的情况下,一种取自游离细胞的人体干细胞实现了无性繁殖。这种快速、定量的方法能导致具有重要临床意义的单细胞的克隆培养,打开了基因控制和多种治疗前景的大门。Daniel Anderson和同事合作,描述了一种可分析数百个聚合物基质的高通量组合阵列方法,以寻找基质表面化合物和干细胞反应之间结构与功能关系。他们发现了可用于无性繁殖的培养基质,从而全面拓展了单个游离人体多能干细胞的功能,并且在没有供给细胞和动物组分的环境中引入了多能干细胞。多能干细胞具有分化成各种细胞类型的能力。在不需额外增加其它成分的环境中培养干细胞,可减少免疫反应。

原文摘要:

Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells

Both human embryonic stem cells and induced pluripotent stem cells can self-renew indefinitely in culture; however, present methods to clonally grow them are inefficient and poorly defined for genetic manipulation and therapeutic purposes. Here we develop the first chemically defined, xeno-free, feeder-free synthetic substrates to support robust self-renewal of fully dissociated human embryonic stem and induced pluripotent stem cells. Material properties including wettability, surface topography, surface chemistry and indentation elastic modulus of all polymeric substrates were quantified using high-throughput methods to develop structure–function relationships between material properties and biological performance. These analyses show that optimal human embryonic stem cell substrates are generated from monomers with high acrylate content, have a moderate wettability and employ integrin αvβ3 and αvβ5 engagement with adsorbed vitronectin to promote colony formation. The structure–function methodology employed herein provides a general framework for the combinatorial development of synthetic substrates for stem cell culture.

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