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10种常用细菌培养基与植物培养基配方

摘要 : Acetobacter Medium (醋酸菌培养基)适用范围:恶臭醋酸杆菌混浊变种。

培养基及成分

1. Acetobacter Medium (醋酸菌培养基)

Glucose (葡萄糖) 100g Yeasst extract (酵母膏) 10g

CaCO3 20g Agar (琼脂) 15g

Distilled water (蒸馏水) 1000ml Adjust (调) pH to 6.8

适用范围:恶臭醋酸杆菌混浊变种

2. Nutrient Agar (营养肉汁琼脂)

Pepton (蛋白胨) 5g Beef extract (牛肉膏) 30g

NaCl 5g Agar (琼脂) 15g

Distilled water (蒸馏水) Adjust (调) pH to 7.0-7.2

[Note]:When cultivation of Bacillus,5mg of to MnSO4.H2O may be added .

It is favorABLe to promote spore formation .

适用范围:产气气杆菌、粪产碱杆菌、蜡状芽孢杆菌、蜡状芽孢杆菌蕈状变种、地衣形芽孢杆菌、巨大芽孢杆菌、多粘芽孢杆菌、尘埃芽孢杆菌、短小芽孢杆菌、嗜热脂肪芽孢杆菌、枯草芽孢杆菌、枯草芽孢杆菌深黑变种、苏云金芽孢杆菌、苏云金芽孢杆菌蜡螟亚种(青虫菌)、苏云金芽孢杆菌戈尔斯德变种、苏云金芽孢杆菌猝倒亚种、产氨短杆菌、黄色短杆菌、谷氨酸棒状杆菌、北京棒杆菌、大肠埃希氏菌(大肠杆菌)、铜绿假单胞菌(绿脓杆菌)、凸形假单胞杆菌、荧光假单胞菌、弯曲假单胞菌、恶臭假单胞菌、假单胞杆菌、藤黄八叠球菌、亚黄八叠球菌、尿素八叠球菌、金黄色葡萄球菌、运动发酵单孢菌。

4. Corn Meal Medium (玉米粉培养基)

Maize flour (玉米粉) 5g Peptone (蛋白胨) 0.1g

Glucose (葡萄糖) 1g Tap water (自来水) 1000ml

[Note]:Boil the mixture in autoclave at 121℃ for 1 hr. distribute the medium into 18ⅹ18 mm tubes , each contains 10 ml of the liquid , then autoclave at 121℃ for 1 hr . again (15磅蒸煮1小时,分装入18ⅹ18毫米试管,每管深度达6厘米。15磅再次灭菌15小时。)

5. Lactic-bacteria Medium I (乳酸菌培养基 I )

Yeast extract (酵母膏) 7.5g Peptone (蛋白胨) 7.5g

Glucose (葡萄糖) 10g KH2PO4 2g

Tomato juice (西红柿汁) 100ml Tween (吐温) 80 0.5ml

Distilled water (蒸馏水) 900ml pH 7.0

适用范围:植物乳杆菌(胚芽乳杆菌)、嗜热乳酸链球菌

6. Lactic-bacteria Midium Ⅱ (乳酸菌培养基 Ⅱ)

Lacto-casein peptone (乳酪蛋白胨) 10g Beef extract (蛋白胨) 10g

Yeast extract (酵母膏) 5g Glucose (葡萄糖) 5g

Tween (吐温) 80 1g K2HPO4 2g

Na-acetate (醋酸钠) 5g Diamine citrate (柠檬酸二胺) 2g

MgSO4.7H2O 0.2g MnSO4.H2O 0.05g

Distilled water (蒸馏水) 1000m pH 6.5-6.8

适用范围:植物乳杆菌(胚芽乳杆菌)

7. Peotone Glucose Yeast extract Medium PGY (蛋白胨、酵母膏、葡萄糖培养基)

Peptone(蛋白胨) 10g Yeast extract (酵母膏) 5g

Glucose (葡萄糖) 1g Distilled water (蒸馏水) 1L

8. Glycerol Agar (甘油琼脂)

Peptone (蛋白胨) 5g Beef extract (酵母膏) 3g

Glycerol (甘油) 20g Top water (自来水) 1000ml

Agar (琼脂) 15g pH 7.0-7.2

9. Rhizobium medium (根瘤菌培养基)AS 9

Yeast eztract (酵母膏) 1g Soil eztract (土壤浸提液) 200ml

Mannitol (甘露醇) 10g Agar (琼脂) 15g

Distilled water (蒸馏水) 800ml pH 7.2

[Note]:Soil extract:Suspend 50g finely and dried gardon soil in 200ml of tap water. Autoclave at 121℃ for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121℃ , then mixed with othringredients and distributed. (土壤浸提液的制法:取土壤50克,加水200毫升,15磅蒸煮1小时,经滤纸过滤后加水补足到200毫升。)

适用范围:大豆根瘤菌(慢生型)、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、

大豆根瘤菌(快生型)、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌

10. Mannitol Agar (甘露醇琼脂)

Yeast extract (酵母膏) 5g Peptone (蛋白胨) 3g

Mannitol (甘露醇) 25g Agar (琼脂) 15g

Distilled water (蒸馏水) 1000ml

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