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PROTOCOLS

标签:WESTERN BLOPROTOCOLS

蛋白提取与溶液配制

[生物化学技术] 2008年05月份

SDS

标签:PROTOCOLSSDS-PAGEFromCurrentprotocolSDSadaptedCh.

SDS-PAGE PROTOCOL Adapted from Current Protocols, Ch. 10 Veena Mandava Materials To Pour Gels: 30% acrylamide 10% SDS 10% APS (make fresh each time) TEMED 1.5 M Tris, pH 8.8 (resolving gel) 1.0 M Tris, pH 6.8 (stacking gel) 5x SDS Running Buffer (1 L) Tri

标签:PROTOCOLStwoSouthernaregiven

Two protocols are given here. The first one is used for BAC library screening on filters , although is also suitable for Transfer hybridizations. PREPARATION OF HYBRIDIZATION SOLUTION Prehybridization solution Final dH2O 1M Tris pH 8.0 0.5M EDTA pH 8.0 20

标签:PROTOCOLSRNAilisted

The protocols listed here are for Drosophila cells in 6 well plates and our pre-aliquoted 384 well plates. RNAi experiments may be done in other size plates, just scale up or down. Adopted from Clemens et al., PNAS 2000 vol 97(12): 6499-6503 I. 6-Well Pla

标签:PROTOCOLSblottingSouthernTMCFBUFFERS

BUFFERS: 1) Prehybridization/hybridization buffer 1% SDS 6X SSC 10 % dextran sulfate 100 mg salmon sperm DNA/ml (added with the radioactive probe) Note: Store hybridization buffer at 4 degrees and warm to 65 degrees prior to use since the dextran sulfate

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