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标签:WesternSyngene凝胶成像

Syngene采用世界上最先进的CCD芯片,具有专门用于化学发光成像的制冷型CCD,可以很好的降低检测时的热噪音。不同大小光圈的镜头与多款CCD合理搭配,保证以最优的硬件配置实现最完美的成像结果。多款大光圈定焦镜头与电动升降载物台的组合,可以实现化学发光成像时样品与CCD之间的近距离接触,减少光信号的损失,保证检测灵敏度。

标签:封闭液Western封闭剂

碧云天生产的Western封闭液(Blocking Buffer)可以用于Western时转移了蛋白样品的PVDF膜或NC膜的封闭。封闭后,可以减小后续的一抗或二抗和膜的非特异性结合,降低背景,增强信噪比。

标签:blottingblotBSAWestern

Blotting with BSA 1. Blot in 5% mile/ 1X PBST (or 1X TBST) --60'/Rt 2. Wash 2X for 5' in 1XPBST 3. 10Ab in 1% BSA/1X PBST --60'/Rt 4. Wash PBST 15', then 3x for 5' each in 1X PBST 5. 20Ab in 1% BSA/1X PBST --60'/Rt (aHRP)-- 6. Wash 15' with 1X PBST then 3

标签:蛋白鉴定印迹Western

实验十二 Western印迹鉴定目标蛋白【实验目的】 1.了解Western blot的原理及其意义,掌握Western blot的操作方法; 2.应用Western blot 技术分析鉴定经SDS-PAGE分离后转移到尼龙膜上的重组蛋白。 【实验原理】 Western印迹法简称蛋白质印迹法。蛋白质样品经 SDS-PAGE电泳后,凝胶所含的样品蛋白质区带通过电泳方法转移、固定到载体(如尼龙膜、硝酸纤维素膜)上,固相载体以非共价键的形式

标签:SDS-PAGEblottingWesternandSDSPAGEConductaccording

Conduct SDS-PAGE according to standard procedures. 1. Cast gel and allow to polymerize 2. Prepare samples to be resolved 3.Load gel 4.Run gel ( 200 volts for ~ 1 – 1.5 hours) Transferring proteins to nitrocellulose membrane. Can either use “wet” or “semid

标签:blotWesternMaterialsSDSAnalysisReadyPrecastGels

Materials Ready Gel Precast Gels Bio-Rad Mini-Protean-3 cell apparatus Prestained protein markers Reagents 6x Laemmli reducing sample buffer Distilled H20 (dH20) Running buffer Cold Transfer buffer Before you start: Turn on heat block to 95-100°C Make sur

标签:blottingWesternmethodDepartmentMichiganState

Department of Medicine, Michigan State University, East Lansing, Michigan, USA. hongbao@msu.edu Abstract: Western blotting is a useful and sensitive method to measure the ng/ml to pg/ml ordered materials in the solution, such as serum, urine and culture s

标签:WesternOfferUsingAdvantagesBlotsEviFluorsmajor

EviFluors offer major advantages over traditional chemiluminescent and radioactive techniques used in Western blot analysis. In addition to offering the ability to color multiplex it also offers a signifi cant cost savings. Some of the advantages of T2-MP

标签:电泳常见问题WesternCommon

表1 Common problems for electrophoresis and western bloting 问 题 可能原因 建议解决方法 推荐电压条件下电泳时间过长。电泳缓冲液过稀。配制新鲜缓冲液,使用1X稀释液。推荐电压条件下电流过高,热量过大。电泳缓冲液过稀。配制新鲜缓冲液,使用1X稀释液。推荐电压条件下电流过低或无电流。接触不良。在电泳前移除胶盒底部的封条;确认缓冲液没过加样孔;检查buffer core上导线连接。

标签:蛋白质ELISAblotWestern

免疫组化实验技术专题: http://ask.bbioo.com/special/experiment/immunohistochemistry.htm 1 原理: 将通过聚丙烯酰胺凝胶电泳分离的蛋白质转移到硝酸纤维素或PVDF膜上,然后与能特异性识别待检蛋白的抗体进行反应,洗涤去除没有结合的特异性抗体后,加入标记的、能识别特异性抗体的种属特异性抗体,反应一段时间后再次洗涤去除非特异性结合的标记抗体,加入适合标记物的检测试剂进行

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