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Plant Physiol:北京大学白书农实验室再次提出植物生物学新概念

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摘要 : 2017年4月,国际植物学顶级期刊《Plant Physiology》杂志上在线发表了北京大学生命科学学院白书农实验室题为Phosphorylation of SPOROCYTELESS/NOZZLE by the MPK3/6 Kinase Is Required for Anther Development的研究论文

2017年4月,国际植物学顶级期刊《Plant Physiology》杂志上在线发表了北京大学生命科学学院白书农实验室题为Phosphorylation of SPOROCYTELESS/NOZZLE by the MPK3/6 Kinase Is Required for Anther Development的研究论文,文章被评为当期封面文章,研究报道一个重要的发现,即在拟南芥中决定生殖细胞分化的SPL基因的功能依赖于其编码蛋白的磷酸化。白书农教授随后发表一篇观点文章在《Plant Signaling and Behavior》杂志上,研究综述题为Two types of germ cells, the sexual reproduction cycle, and the double-ring mode of plant developmental program,提出由于植物自养所决定的独特的形态建成策略,植物完成生活周期需要两次生殖细胞分化,从而出现两类“生殖细胞(germ cells)”,一类是二倍体生殖细胞(diploid germ cells, DGC),一类是单倍体生殖细胞(haploid germ cells, HGC)。这与动物生活周期完成过程中只出现过一次生殖细胞分化,通过生殖细胞系(germline)作为有性生殖周期载体的情况不同。Plant Physiol.文章的第一作者是白书农实验室博士研究生赵峰。

植物生物学研究中,由于历史的原因,为了解释现象提出过很多似是而非的概念。在过去以解剖和生理为主的研究阶段,这些概念从技术上无法澄清,同时对研究工作的发展也不产生特别的困扰,因此一直代代相传。可是随着分子技术所带来的研究工具分辨力大幅度提高,这些似是而非的概念对研究的深入就带来各种无法回避的逻辑困境。基于过去的研究工作中,白书农曾经先后提出过“植物发育单位”、有性生殖周期(SRC)、植物发育程序的双环模式等不同的新概念和“植物形态建成123”的新理论,希望对更好地理解植物形态建成的内在机制提供更为合理的概念框架。从影响因子的角度,好像只有plant Physiol之类杂志以上的文章才更多地被推介。可是,从科学意义的角度,两类生殖细胞的概念应该有更大的价值,值得搭Plant Physiol.的文章的便车向大家推介。其他的概念因为没有便车可搭,过去也就没有机会推介。但有兴趣的师生可以关注参考文献。

Plant Physiol:北京大学白书农实验室再次提出植物生物学新概念
重新构建的PSPL: GUS报告基因在mpk突变体的转基因植株花中表现出雄蕊和胚珠特异性

原文链接:

Phosphorylation of SPOROCYTELESS/NOZZLE by the MPK3/6 Kinase Is Required for Anther Development

原文摘要:

Germ cells are indispensable carriers of genetic information from one generation to the next. In contrast to the well-understood process in animals, information on the mechanism of germ cell initiation in plants is very limited. SPOROCYTELESS/NOZZLE was previously identified as an essential regulator of diploid germ cell (archesporial cell) differentiation in the stamens and ovules of Arabidopsis (Arabidopsis thaliana). Although SPOROCYTELESS (SPL) transcription is activated by the floral organ identity regulator AGAMOUS and epigenetically regulated by SET DOMAIN GROUP2, little is known about the regulation of the SPL protein. Here, we report that the protein kinases MPK3 and MPK6 can both interact with SPL in vitro and in vivo and can phosphorylate the SPL protein in vitro. In addition, phosphorylation of the SPL protein by MPK3/6 is required for SPL function in the Arabidopsis anther, as measured by its effect on archesporial cell differentiation. We further demonstrate that phosphorylation enhances SPL protein stability. This work not only uncovers the importance of SPL phosphorylation for its regulatory role in Arabidopsis anther development, but also supports the hypothesis that the regulation of precise spatiotemporal patterning of germ cell initiation and that differentiation is achieved progressively through multiple levels of regulation, including transcriptional and posttranslational modification.

doi:10.1104/pp.16.01765

作者:白书农 点击:

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