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Plant Physiol:南京农业大学万建民研究组发表水稻茎尖分生组织调控机制研究论文

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摘要 : 近日,国际植物学顶级期刊《plant Physiology》杂志上在线发表了中国农业科学院作物科学研究所作物功能基因组研究创新团队、南京农业大学万建民院士研究组在水稻分生组织调控机制研究突破论文

近日,国际植物学顶级期刊《plant Physiology》杂志上在线发表了中国农业科学院作物科学研究所作物功能基因组研究创新团队、南京农业大学万建民院士研究组在水稻分生组织调控机制研究突破论文,研究首次鉴定到关键信号基因Argonaute10的转录调控因子LBD12-1,研究文章题为“The LBD12-1 transcription factor suppresses apical meristem size by repressing Argonaute 10 expression”。博士生马炜炜和吴赴清副研究员为论文共同第一作者,万建民院士为通讯作者。

对高等植物而言,茎尖分生组织是所有地上部分的起源,水稻茎尖分生组织决定了株高、穗长、分蘖数等重要农艺性状。植物分生组织受多条信号通路的调控,其中Argonaute家族基因AGO1和AGO10通过调控下游HD-ZIP III基因的表达水平和表达模式,在维持植物分生组织活性和侧生器官发育上发挥重要作用,是植物分生组织调控网络中的重要节点。但到目前为止,AGO10基因自身的表达调控机制还未见报道。

万建民院士团队研究发现,过量表达转录因子LBD12-1引起水稻株高降低并伴随叶片和花药发育异常。盐胁迫下LBD12-1表达上升,并直接结合在AGO10启动子上抑制其转录,从而阻碍茎尖分生组织的生长。该研究加深了对植物茎尖分生组织调控机制的理解,具有重要科学意义。

Plant Physiol:南京农业大学万建民研究组发表水稻茎尖分生组织调控机制研究论文
水稻茎尖分生组织的调控机制示意图

原文链接:

The LBD12-1 transcription factor suppresses apical meristem size by repressing Argonaute 10 expression

原文摘要:

The shoot apical meristem (SAM) consists of a population of multipotent cells that generate all aerial structures and regenerates itself. SAM maintenance and lateral organ development are regulated by several complex signaling pathways, in which the Argonaute gene mediated pathway plays a key role. One Argonaute gene, AGO10, functions as a miRNA locker that attenuates miR165/166 activity and positively regulates shoot apical meristem development, but little is known about when and how AGO10 is regulated at the transcriptional level. In this research we showed that transgenic rice plants overexpressing LBD12-1, an LBD family transcription factor, exhibited stunted growth, twisted leaves, abnormal anthers, and reduced SAM size. Further research revealed that LBD12-1 directly binds to the promotor region and represses the expression of AGO10. Overexpression of AGO10 in an LBD12-1 overexpression background rescued the growth defect phenotype of LBD12-1 overexpressing plants. The expression of LBD12-1 and its binding ability to the AGO10 promoter is induced by stress. lbd12-1 loss-of-function mutants showed similar phenotypes and SAM size to WT under normal conditions, but lbd12-1 had a larger SAM under salt stress. Our findings provide novel insights into the regulatory mechanism of AGO10 by which SAM size is controlled under stress conditions.

doi:10.1104/pp.16.01699

作者:万建民 点击:

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